Parameters

MET

GLZ

PLS-1

NAP/CLS

PLS-1

NAP/CLS

Calibration set results

No. of factors

9

11

6

6

Press

0.2066

0.3082

0.0434

0.0190

RMSE(µg/ml)

0.1136

0.1388

0.0520

0.0345

REP%

0.7410

0.9051

1.6743

1.1102

Slope

1.0035

0.9996

1.0052

1.0018

R2

0.9996

0.9993

0.9994

0.9992

Bias

0.0000

0.0000

0.000

0.0000

Validation set results

Press

1.6127

1.5232

0.0723

0.0661

RMSE(µg/ml)

0.4489

0.4364

0.0951

0.0909

REP%

3.2070

3.1169

3.1696

3.0292

Slope

1.0686

1.0625

1.0438

1.0382

R2

0.9912

0.9911

0.9966

0.9976

Bias

-0.0349

0.0367

0.0225

0.0463

Figure of merits

LOD(µg/ml)

0.0965

0.1183

0.0441

0.0294

LOQ(µg/ml)

0.2923

0.3584

0.1337

0.0890

SEM

0.0293

0.035

0.0134

0.0089

Table 3: Statistical parameters for the optimised models.