Figure 3: The effect of β-thujaplicin on the promoter activities of 5’-upstream regions of human shelterin encoding genes. Reporter plasmids (10 ng) and DEAE-dextran were spotted and dried onto each well of the 96-well culture plate. HeLa-S3 cells (1 x 105/well) were used for transfection and incubated for further 24 h, then treated with β-thujaplicin (10 μM) for 24 h. Results show relative Luc activities from various Luc reporter transfected cells compared with that of pGL4-PIF1 transfected cells.