Figure 1: 3D electropherogram of (a) standard heparin and (b) force degraded heparin under thermal stress after 720 hours. According to the 3D plot, the highest sensitivity of the method for heparin was at 200 nm and for force degradation products was at 257 nm. Separation conditions: 50 μm i.d.; 50 cm total and 41.5 cm effective length, buffer phosphate 72 mM, pH=3.50, cassette temperature :35°C, applied voltage: -30 kV, hydrodynamic injection at 50 mbar for 40 seconds.