Figure 2: Electropherogram of heparin standard solution under optimized conditions. Separation conditions: capillary 50 μm i.d. (50 cm total and 41.5 cm effective length), electrolyte consisting of buffer sodium phosphate 72 mM, pH=3.5, temperature: 35°C, applied voltage: -30 kV and hydrodynamic injection 50 mbar for 40 seconds.