Calibration range Calibration equation Regression
coefficient
LOQ1 LOD2
0.312 – 15.0 mg/ml y = 77.10x + 0.189 0.998 0.312 mg/ml 0.078 mg/ml
*Separation conditions: capillary 50 μm i.d. (50 cm total and 41.5 cm effective length), buffer phosphate 72 mM, pH=3.50, temperature: 35°C, voltage: -30 KV.
1. Limit of quantification
2. Limit of detection
Table 1: Accuracy and precision of the method.