Figure 1: Schematic diagram of a representative setup of LC coupled to post column derivatization; MP = LC mobile phase; IV = LC high pressure injection valve (or alternatively an autosampler); AC = analytical column (reversed phase, ion exchange, hydrophilic interaction etc); PP = propulsion pump of the derivatization reagent(s); R1/R2= post column derivatization reagents; RC = reaction coil (thermostated depending on the application); UV = detector; W = waste.