Figure 4: Deformation of bovine serum albumin (BSA) by nitrogen gas plasma, which was confirmed by secondary electrophoresis.
Each arrow shows the original size of BSA. Compared with the one band of control BSA (left), exposed BSA by nitrogen gas plasma (right) is degraded and fragmentary. Some fragments may occur polymerization. Anyhow, deformation of BSA is occurred by nitrogen gas plasma.
Material: Bovine serum albumin (BSA) 500 ng Method: Secondary SDS Poly-Acrylamide Gel Electrophoresis Low pressure nitrogen gas plasma reactor: Anode-Cathode Gap: 40mm, Vac: 43000 Pa, nitrogen gas flow speed: 6 L/min, Time: 30min, Temperature: 60°C (Temperature was measured inside of plasma) (Electric Source) Input Power: 84W, Rep. Freq. 2.5kHz, Peak Voltage:19.0 kV.