Parameter D1 method DD1 method MCN method HPLC method 290.4 nm 332 nm 288.5 nm 328 nm Range 5-30 µg mL-1 5-30 µg mL-1 5-30 µg mL-1 2-20 µg mL-1 Slope 0.0097 0.0120 0.0992 0.1248 0.0530 0.1334 Intercept 0.0011 -0.0047 -0.0015 -0.0305 -0.0039 -0.0318 SE of the slope 8.463 × 10-5 8.443 × 10-5 0.0010 0.0014 0.0001 0.0009 SE of the intercept 0.0016 0.0016 0.0193 0.0271 0.0021 0.0113 Correlation coefficient( r ) 0.9998 0.9999 0.9998 0.9997 1 0.9998 LOD 0.5364 0.4345 0.6178 0.6894 0.1281 0.3884 LOQ 1.6255 1.3167 1.8798 2.0889 0.3881 1.1769 Accuracy (mean ± RSD %) 99.86 ± 1.249 99.98 ± 0.868 100.11 ± 1.020 100.15 ± 1.043 100.09 ± 0.387 100.00 ± 1.302 Precision Repeatability % Intermediate precision % 0.847 0.977  0.853 0.733 0.508 0.615 0.582 0.743 0.631 0.584  0.935 0.749

Table 1: Validation parameters of the proposed method for the determination of OZ pure samples.