Parameters OZ Deg limit
Retention time (tR) 4.298 3.050  
Resolution ( R ) 5.16   Rs >2
Tailing factor (T) 1.17   T < 2
Capacity factor (K') 9.745 6.625 1< K' <10
Selectivity factor (α) 1.471   α > 1
Column efficiency (N) 9552.59 1575.114 N > 2000
Height equivalent to theoretical plate (HETP) 0.026 0.159  
Table 2: System suitability parameters of the proposed HPLC method.