Parameter |
D1 method |
DD1 method |
MCR method |
HPLC method |
**Reported HPLC method |
At 290.4 nm |
At 332 nm |
At 288.5 nm |
At 328 nm |
Mean |
99.9 |
100.86 |
100.62 |
99.88 |
98.40 |
100.97 |
99.78 |
SD |
1.031 |
0.962 |
0.455 |
0.500 |
1.185 |
0.509 |
0.852 |
n |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
Variance |
1.063 |
0.925 |
0.207 |
0.250 |
1.404 |
0.259 |
0.726 |
Student's t |
0.137 (2.45)* |
1.284 (2.45)* |
1.328 (2.45)* |
0.155 (2.45)* |
1.44 (2.45)* |
1.028 (2.45)* |
|
F |
1.464
(9.2766)* |
1.274 (9.2776)* |
3.507
(9.2766)* |
2.904
(9.2776)* |
1.934 (9.2776)* |
2.803
(9.2776)* |
|
* The values in parentheses are the corresponding tabulated values at p=0.05.
** HPLC method (C-18, using acetonitrile: water 38:62 v/v consisting of triethylamine and adjusted to pH 3.6 with 5% O-phosphoric acid, flow rate 0.8 mL min1, 254 nm). |