HPLC
D1
DD1
iso
offecial method
taken
found
recovery
taken
found
recovery
found
recovery at 225
found
recovery at 231.5
found
recovery at 237.5
50
51.27
102.54
4
4.04
101.04
4.01
100.25
4.03
100.66
4.11
102.63
100
99.22
99.22
8
7.79
97.43
8.12
101.55
8.04
100.47
8.04
100.56
200
202.48
101.24
12
12.12
101.04
12.06
100.52
11.97
99.77
11.90
99.20
300
296.09
98.7
16
16.17
101.04
16.08
100.5
15.91
99.42
15.87
99.19
400
404.24
101.06
20
19.92
99.6
20.14
100.7
19.99
99.97
19.92
99.59
500
500.7
100.14
24
23.96
99.84
24.27
101.1
24.08
100.34
24.10
100.42
28
28
100
28.18
100.63
27.99
99.98
28.07
100.24
X-
100.58
100.48
99.99
100.48
100.09
100.26
±SD
0.805
1.421
1.293
0.437
0.430
1.187
%RSD
0.800
1.414
1.239
0.435
0.428
1.184
N
6
7
7
7
7
t
1.172
0.592
0.011
0.0235
0.244
F
2.494
3.172
1.741
4.395
3.268
Table 2:
Determination and statistical analysis of the results obtained for assay of authentic Fluticasone propionate using, HPLC, D1, DD1 and isosbestic points methods compared with official method.