HPLC     D1     DD1       iso  
    offecial method taken found recovery taken found recovery found recovery at 225 found recovery at 231.5 found recovery at 237.5
    50 51.27 102.54 4 4.04 101.04 4.01 100.25 4.03 100.66 4.11 102.63
    100 99.22 99.22 8 7.79 97.43 8.12 101.55 8.04 100.47 8.04 100.56
    200 202.48 101.24 12 12.12 101.04 12.06 100.52 11.97 99.77 11.90 99.20
    300 296.09 98.7 16 16.17 101.04 16.08 100.5 15.91 99.42 15.87 99.19
    400 404.24 101.06 20 19.92 99.6 20.14 100.7 19.99 99.97 19.92 99.59
    500 500.7 100.14 24 23.96 99.84 24.27 101.1 24.08 100.34 24.10 100.42
          28 28 100 28.18 100.63 27.99 99.98 28.07 100.24
X- 100.58     100.48     99.99   100.48   100.09   100.26
±SD 0.805     1.421     1.293   0.437   0.430   1.187
%RSD 0.800     1.414     1.239   0.435   0.428   1.184
N       6     7   7   7   7
t       1.172     0.592   0.011   0.0235   0.244
F       2.494     3.172   1.741   4.395   3.268
Table 2: Determination and statistical analysis of the results obtained for assay of authentic Fluticasone propionate using, HPLC, D1, DD1 and isosbestic points methods compared with official method.