HPLC       Spectro   recovery            
    taken   recovery   taken   D1   DD1     iso  
mix SAM : FLU ratio SAM FLU SAM FLU SAM FLU SAM FLU SAM at 334 SAM at 337.5 FLU at 231.5 SAM at 343 FLU 237.5
1 1::1 100 100 101.53 100.52 12 12 99.54 101.04 98.14 100.37 101.05 99.44 101.84
2 1::3 100 300 100.93 100.58                  
3 1::2 100 200 101.19 99.61 8 16 99.54 102.48 101.98 102.07 100.85 101.18 100.20
4 3::2 300 200 100.79 101.58 12 8 102.10 101.04 98.93 99.87 100.23 101.65 99.53
5 2::3 200 300 101.25 99.85 8 12 99.72 101.04 100.60 98.17 99.61 100.23 100.24
6 2::5         8 20 99.72 101.04 102.33 98.70 100.26 99.52 100.66
7 4::3         16 12 101.30 101.04 98.14 100.37 101.23 100.26 100.24
8 4::5         8 20 101.43 101.48 102.08 101.97 100.26 99.40 101.01
x-       100.82 100.74     100.48 101.31 100.31 100.22 100.49 100.24 100.53
±SD       0.738 0.665     1.089 0.542 1.889 1.481 0.568 0.889 0.735
%RSD       0.732 0.660     1.084 0.535 1.883 1.478 0.565 0.887 0.731
Table 5: Determination of SAM and Fluticasone propionate in laboratory prepared mixtures by the proposed methods.