Cell lines Growth medium Atmosphere Growth temp. Growth condition Storage Hep G2 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 2.5mM L-glutamine, 15mM HEPES, 0.5mM sodium pyruvate, 1200mg/l sodium bicarbonate, 100µg/ml penicillin and streptomycin, 10% FBS 95% air ; 5% CO2 37°C Add fresh medium every 4 to 5 days (depending on cell density) Complete growth medium supplemented with 5% (v/v) DMSO ; liquid nitrogen HeLa Eagle's Minimum Essential Medium containing 100µg/ml penicillin and streptomycin, 10% FBS 95% air ; 5% CO2 37°C Add fresh medium every 2 to 4 days (depending on cell density) Complete growth medium supplemented with 5% (v/v) DMSO ; liquid nitrogen MCF7 Eagle's Minimum Essential Medium containing 0.01 mg/ml bovine insulin; 100µg/ml penicillin and streptomycin, 10% FBS 95% air ; 5% CO2 37°C Add fresh medium every 2 to 4 days (depending on cell density) Complete growth medium supplemented with 5% (v/v) DMSO ; liquid nitrogen Sp2/0-Ag14 Dulbecco's Modified Eagle's Medium containing 100µg/ml penicillin and streptomycin, 10% FBS 95% air ; 5% CO2 37°C Add fresh medium every 2 to 4 days (depending on cell density) Complete growth medium supplemented with 5% (v/v) DMSO ; liquid nitrogen

Table 1: Growth parameters of cell lines used to assay in vitro cytotoxocity of native and rec-pediocin.