| Parameter |
Method A |
Method B |
| λmax, nm |
225 |
225 |
| Linear range, µg mL-1 |
0.5-5.0 |
1.25-12.5 |
| Molar absorptivity(ε), L mol-1cm-1 |
8.65×104 |
2.11×104 |
| Sandell sensitivity*, µg cm-2 |
0.003 |
0.0121 |
| Limit of detection (LOD), mgmL-1 |
0.01 |
0.03 |
| Limit of quantification (LOQ), mgmL-1 |
0.02 |
0.09 |
| Regression equation, Y** |
| Intercept (a) |
0.0161 |
-0.0094 |
| Slope (b) |
0.3311 |
0.0849 |
| Standard deviation of a (Sa) |
0.0998 |
0.0998 |
| Standard deviation of b (Sb) |
0.032 |
0.0124 |
| Variance (Sa2) |
0.01 |
0.01 |
| Regression coefficient (r) |
0.9999 |
0.9999 |
*Limit of determination as the weight in μg per mL of solution, which corresponds to
an absorbance of A=0.001 measured in a cuvette of cross-sectional area 1 cm2
and l=1 cm.
**Y=a+bX, Where Y is the absorbance, X is concentration in μgmL-1, a is intercept,
b is slope. |
