**Parameter** |
**Method A** |
**Method B** |
λ_{max}, nm |
225 |
225 |
Linear range, µg mL^{-1} |
0.5-5.0 |
1.25-12.5 |
Molar absorptivity(ε), L mol^{-1}cm^{-1} |
8.65×10^{4} |
2.11×10^{4} |
Sandell sensitivity*, µg cm^{-2} |
0.003 |
0.0121 |
Limit of detection (LOD), mgmL^{-1} |
0.01 |
0.03 |
Limit of quantification (LOQ), mgmL^{-1} |
0.02 |
0.09 |
Regression equation, Y** |
Intercept (a) |
0.0161 |
-0.0094 |
Slope (b) |
0.3311 |
0.0849 |
Standard deviation of a (S_{a}) |
0.0998 |
0.0998 |
Standard deviation of b (S_{b}) |
0.032 |
0.0124 |
Variance (Sa^{2}) |
0.01 |
0.01 |
Regression coefficient (r) |
0.9999 |
0.9999 |
*Limit of determination as the weight in μg per mL of solution, which corresponds to
an absorbance of A=0.001 measured in a cuvette of cross-sectional area 1 cm^{2}
and l=1 cm.
**Y=a+bX, Where Y is the absorbance, X is concentration in μgmL^{-1}, a is intercept,
b is slope. |