Figure 5: Typical densitogram of simulated Placebo mixture of excipients used as a blank by using a mobile phase containing toluene: acetone: glacial acetic acid (10:15:0.2 v/v/v), saturation time of 20 min using 5 cm×10 cm TLC silica gel 60 F254 plate and application volume was 5 μL at a detection wave length of 284 nm.