Figure 6: Typical densitograms obtained during method specificity test (1) Placebo mixture of excipients in methanol (2) methanol and (3) diclofenac sodium. The chromatographic conditions consisted of mobile phase toluene: acetone: glacial acetic acid (10:15:0.2v/v/v) at a saturation time of 25 min, on a 20 cm×10 cm TLC silica gel 60 F254 plate with application volume of 5 μL, and detection at 284 nm. Diclofenac sodium (0.2 mg/mL) had a Rf of 0.60. |