Validation Parameters Ethinyl estradiol Drospirenone
Mobile Phase

70:30 (Ammonium Acetate: ACN)

70:30 (Ammonium Acetate: ACN)

Flow Rate 1 ml/min 1 ml/min
Detection wave Length PDA at 258 nm PDA at 258 nm
Rt 1.438 5.321
Run Time 7 min 7 min
Asymmetry 1.693 1.188
Theoretical Plates 3636 7728
LOD 0.00026 ppm 0.0925 ppm
LOQ 0.00087 ppm 0.308 ppm
Linearity R2=0.998 R2=0.999
Precision % RSD < 2 % RSD < 2
Recovery 99-101% 98-102%
Table 7: System suitability parameters for HPLC.