Time (sec) Regression equation Correlation coefficient Linear range (µg/mL) 200 A = 0.023C - 0.032 0.9742 2-20 400 A = 0.028C - 0.031 0.9895 2-20 600 A = 0.029C - 0.025 0.9966 2-20 800 A = 0.029C - 0.022 0.9998 2-20 1000 A = 0.029C - 0.024 0.9969 2-20 A-absorbance; C-concentration

Table 3: Regression equations for AVS at fixed time and 25°C.