Parameters TLC HPLC Range 0.2-2 µg/band 10-100 µg mL-1 Linearity Slope Intercept 0.8154 0.1886 0.0526 -0.0234 SE of slope 0.005389 0.000142 SE of intercept 0.007163 0.016 Correlation coefficient (r) 0.9999 1 Accuracy(Mean± SD) 100.11 ± 0.516 100.44 ± 0.722 LOD* 0.0340 (μg/band) 1.5928 (μgmL-1) LOQ* 0.1031 (μg/band) 4.8269 (μgmL-1) Precision (RSD %) Repeatabilitya Intermediate precisionb 1.187 0.053 0.815 0.135 Specificity 100.53 ± 0.534 99.89 ± 0.978 *LOD: Limit of detection, LOQ: Limit of quantitation *LOD = (SD of the response/ slope) × 3.3, LOQ = (SD of the response/ slope) × 10 aThe intraday of samples of BFM (0.1,1and1.4μg/band) for TLC and (40, 80 and 160 μgm-1) for HPLC bThe inter-day of samples of BFM (0.1,1and1.4μg/band) for TLC and (40, 80 and 160 μgm-1) for HPLC

Table 1: Results of regression and validation parameters of the proposed chromatographic methods for determination of Buflomedilin presence of its acid induced degradation products.