Parameters TLC HPLC
Range 0.2-2 µg/band 10-100 µg mL-1
Linearity
Slope
Intercept
0.8154
0.1886
0.0526
-0.0234
SE of slope 0.005389 0.000142
SE of intercept 0.007163 0.016
Correlation coefficient (r) 0.9999 1
Accuracy(Mean± SD) 100.11 ± 0.516 100.44 ± 0.722
LOD* 0.0340 (μg/band) 1.5928 (μgmL-1)
LOQ* 0.1031 (μg/band) 4.8269 (μgmL-1)
Precision (RSD %)
Repeatabilitya
Intermediate precisionb
1.187
0.053
0.815
0.135
Specificity 100.53 ± 0.534 99.89 ± 0.978
*LOD: Limit of detection, LOQ: Limit of quantitation
*LOD = (SD of the response/ slope) × 3.3, LOQ = (SD of the response/ slope) × 10
aThe intraday of samples of BFM (0.1,1and1.4μg/band) for TLC and (40, 80 and 160 μgm-1) for HPLC
bThe inter-day of samples of BFM (0.1,1and1.4μg/band) for TLC and (40, 80 and 160 μgm-1) for HPLC
Table 1: Results of regression and validation parameters of the proposed chromatographic methods for determination of Buflomedilin presence of its acid induced degradation products.