Parameter BFM BFM Deg. Reference value
Retention time(tR) 3.76 2.117  
Resolution (Rs) 4.69 Rs >0.8
Tailing factor (T) 1.33 1.67 T = 1*
Capacity factor (K') 6.52 3.23 K' >2
Selectivity factor (α) 2.02 α >1
Column efficiency (N) 3599 2906 N >2000
Height equivalent to theoretical plate (HETP) 0.016 0.019 AS HETP increase the column efficiency decrease
*For a typical symmetrical peak
Table 4: Statistical analysis of parameters required for system suitability for HPLC.