Parameters |
GMF |
MXF |
Method A |
Method B |
Method C |
Method A |
Method B |
Method C |
λ max (nm) |
510 |
528 |
610 |
510 |
528 |
610 |
Beer’s law limits, µg mL-1 |
0.1-2.0 |
0.1-2.0 |
0.2-4.8 |
0.2-2.4 |
0.2-4.0 |
0.2-2.6 |
Ringboom limits, µg mL-1 |
0.3-1.8 |
0.3-1.8 |
0.5-4.0 |
0.4-2.0 |
0.5-3.6 |
0.4-2.2 |
Molar absorptivity, x 105 L mol-1 cm-1 |
8.25 |
10.31 |
6.49 |
7.17 |
7.79 |
10.66 |
Sandell sensitivity, ng cm-2 |
5.88 |
4.71 |
7.48 |
6.11 |
5.62 |
4.12 |
Regression equationa |
|
|
|
|
|
|
Intercept (a) |
- 0.002 |
0.0008 |
0.0045 |
- 0.0016 |
0.0004 |
- 0.0085 |
Standard deviation of intercept (Sa) |
0.0055 |
0.0058 |
0.0079 |
0.0067 |
0.0091 |
0.0074 |
Slope (b) |
0.1757 |
0.213 |
0.1276 |
0.1669 |
0.1759 |
0.2601 |
Standard deviation of slope (Sb) |
0.0054 |
0.0087 |
0.0094 |
0.0090 |
0.0098 |
0.0086 |
Correlation coefficient, (r) |
0.9997 |
0.9998 |
0.9995 |
0.9997 |
0.9998 |
0.9994 |
Mean ± SD |
100.03 ± 0.844 |
100.18 ± 0.96 |
100.28 ± 0.76 |
99.44 ±
1.49 |
99.90 ±
1.04 |
99.45 ±
1.15 |
RSD% |
0.844 |
0.96 |
0.76 |
1.50 |
1.04 |
1.16 |
RE% |
0.886 |
1.01 |
0.80 |
1.57 |
1.09 |
1.21 |
Limit of detection, µg mL-1 |
0.026 |
0.029 |
0.058 |
0.059 |
0.055 |
0.056 |
Limit of quantification, µg mL-1 |
0.087 |
0.097 |
0.193 |
0.197 |
0.183 |
0.187 |
Calculated t-valueb |
0.13 |
0.39 |
0.64 |
0.65 |
0.07 |
0.76 |
Calculated F-valueb |
1.06 |
1.37 |
1.16 |
2.99 |
1.46 |
1.78 |
aA = a + bC, where C is the concentration in μg mL-1, A is the absorbance units, a is the intercept, b is the slope.
bThe theoretical values of t and F are 2.57 and 5.05, respectively at confidence limit at 95% confidence level and five degrees of freedom (p=0.05). |