Drugs Conc. μgmL-1) Regression Equation r2 LLOD(ngmL-1) LLOQ(ngmL-1)
Bulk
HCT 2.5-100 y = 2489.4x + 3255.5 0.9996 1.6 5
ENP 2.5-100 y = 1970.7x + 2357.9 0.9995 1.53 4.6
FRS 2.5-100 y = 1982.5x + 1478.4 0.9994 4.1 12.6
serum
HCT 2.5-100 2489.4x + 3254.9 0.9996 3.4 10.5
ENP 2.5-100 y = 1970.5x + 2372.9 0.9995 4.7 14.4
FRS 2.5-100 y = 1982.5x + 1481.1 0.9994 6.9 20.1
HCT: hydrochlorothiazide; ENP: enalapril; FRS: furosemide; LLOD: Lower limit of detection; LLOQ: Lower limit of quantification
Table 2: Regression characteristics of the proposed method for hydrochlorothiazide, enalapril and furosemide.