Conc. μgmL-1 HCT  ENP FRS
%Recovery %RSD %Recovery %RSD %Recovery %RSD
2.5 100 0 100 0 100 0
5 100 0.1 100 0.1 100 0.5
10 101 0.2 101 0.4 101 0.2
15 11.2 0.01 11.2 0 11.2 0
25 100.02 0.4 100.02 0.1 100.02 0.7
50 100 0.2 100 0.6 100 0.2
100 101 0.5 101 0.2 101 0.3
Assay (spiking method)
80 100 0.1 100 0.1 100 0.1
100 100.2 0.2 100.2 0.2 100.2 0.1
120 100.3 0.1 100.3 0.1 100.3 0.1
Assay in serum
80 100.2 0.1 100 0.1 100 0.1
100 100 0.2 100.2 0.2 100.2 0.1
120 100.2 0.2 100.3 0.1 100.3 0.2
HCT: hydrochlorothiazide; ENP: enalapril; FRS: furosemide
Table 3: Accuracy of the proposed method for hydrochlorothiazide, enalapril and furosemide.