Conc.μgmL-1 Inter-day Intra-day HCT ENP FRS HCT ENP FRS %RSD %RSD %RSD %RSD %RSD %RSD 2.5 0 0 0 0 0 0 5 0.1 0.1 0.1 0.1 0.1 0.1 10 6 0.2 0.2 0.2 0.2 0.2 15 0 0 0 0 0 0 25 6 0.4 0.4 0.2 0.4 0.4 50 0.2 0.2 0.2 0.2 0.2 0.2 100 0.5 0.2 0.5 0.2 0.5 0.5 Assay in serum 80 0.3 0.1 0.2 0.2 0.1 0.2 100 0.2 0.2 0.2 0.7 0.2 0.2 120 0.6 0.1 0.2 0.2 0.6 0.2 HCT: hydrochlorothiazide; ENP: enalapril; FRS: furosemide

Table 4: Inter and intra-day precision of the proposed method for hydrochlorothiazide, enalapril and furosemide (n=6).