| |
Level |
K’ |
T |
(Rs) |
| A: pH of mobile phase |
| 2.8 |
-0.2 |
17.3 |
1.71 |
5.3 |
| 3 |
0 |
17.7 |
1.73 |
5.5 |
| 3.2 |
0.2 |
17.2 |
1.78 |
5.35 |
| B: Flow rate (mLmin-1) |
| 0.8 |
-0.2 |
17.1 |
1.71 |
5.32 |
| 1 |
0 |
17.7 |
1.73 |
5.5 |
| 1.2 |
0.2 |
17.5 |
1.78 |
5.37 |
| C: Percentage of water in mobile phase (V/V) |
| 80/20 |
-5 |
17.1 |
1.70 |
5.39 |
| 75/25 |
0 |
17.7 |
1.73 |
5.5 |
| 70/30 |
+5 |
17.5 |
1.70 |
5.32 |
| D: Wavelength (nm) |
| 220 |
-5 |
17.7 |
1.72 |
5.39 |
| 225 |
0 |
17.7 |
1.73 |
5.5 |
| 230 |
5 |
17.1 |
1.79 |
5.32 |
K’= Capacity factors, T = Tailing factor, Rs = Resolution |