S. No. Type of Media Column Mobile Phase Detector Chromatographic conditions Reference
1. Human serum YMC ODS (23 × 4.0 mm) YMC ODS (150 × 4.6 mm) Phase A: Acetonitrile and potassium dihydrogen phosphate (20 mM) in ratio of 60:40, pH 4.0
Phase B: Acetonitrile and dihydrogen phosphate (20 mM) in ratio of 60:40, pH 6.0  
UV (229 nm) Flow rate: 1.0 mL.min-1 Temperature: 40°C [36]
2. Human serum and urine Inertsil ODS-2 ( 150 × 4.6 mm, 5μm) First column: 0.02 M potassium  dihydrogen phosphate Acetonitrile (40:60) adjusted to pH 4.0 with 85 orthophosphoric acid
Second column: 0.02 M potassium  dihydrogen phosphate Acetonitrile (40:60) adjusted to pH 6.0 with 1M NaOH
Fluorescence detector (Excitation and Emission wavelengths set at 270 and 390 nm) Flow rate: 1.0 mL.min-1 Temperature: 40°C [37]
3. Human plasma and urine Spherisorb S3P (100 × 4.6 mm, 3 μm Hichrom) Candesartan (Plasma):
100 ml citrate buffer (pH 3.1, I=0.5 containing 50 mM TBA), 185 ml acetonitrile, 180 ml methanol and diluting to 1000 ml with water
Fluorescence detector (Excitation and Emission wavelengths set at 265 and 395 nm) Flow rate: 0.9 mL.min-1 Temperature: room [40]
Candesartan cilexetil (Plasma & urine):
200 ml phosphate buffer (pH 2.8, I=0.1 containing 12.5 mM TBA), 420 ml acetonitrile and diluting to 1000 ml with water
Flow rate: 1.0 mL.min-1 Temperature: room
Candesartan (Urine):
Phase A: 200 ml phosphate buffer (pH 2.8, I=0.1 containing 12.5 mM TBA), 200 ml acetonitrile and diluting to 1000 ml with water
Phase B: 200 ml phosphate buffer (pH 2.8, I=0.1 containing 12.5 mM TBA), 600 ml acetonitrile and diluting to 1000 ml with water
Flow rate: 1.0 mL.min-1 Temperature: room
4. Human plasma Novapak guard column (20 × 39 mm, 4 μm) muBondapak  (300 × 3.9 mm, 10 μm) Acetonitrile (5 mM) acetate buffer (pH 4) Fluorescence detector (Excitation and Emission wavelengths set at 250 and 375 nm) Flow rate: 1.0 to 1.2 mL.min-1 Temperature: room [41]
5. Human plasma and urine Betasil (250mm × 4.6mm, 5μm) Acetonitrile and  Sodium acetate buffer solution (5mM, pH 3.5) in the ratio of 40:60) PDA (250 nm) and fluorescence  (Excitation and Emission wavelengths set at 250 and 380 nm) Flow rate: 1.0 mL.min-1 [42]
6. In vitro dissolution Inertsil ODS-3 (250 × 4.6-mm, 5 μm) 0.02 M monobasic potassium phosphate, acetonitrile, and triethylamine in the ratio of 40:60:0.2 (pH adjusted to 6.0 using phosphoric acid) UV (254 nm) Flow rate: 2.0 mL.min-1 Temperature: 25°C [43]
7. Stability studies Hypersil ODS (250 × 4.6 mm, 5 μm) 0.02M Potassium dihydrogen phosphate solution and acetonitrile (2:8), pH 4.0 was adjusted with 85 phosphoric acid UV (254 nm) Flow rate: 1.0 mL.min-1 Temperature: ambient [44]
8. Drug analysis Zorbax SB-Phenyl 0.1 mol.L-1 sodium acetate (pH 5.5), acetonitrile and methanol in 10:9:6 v/v/v ratio UV (230 nm)   [45]
9. Drug analysis Hypersil ODS (250 × 4.6 mm, 5 μm) Tetra butyl ammonium hydrogen sulphate 10 mM (pH 3.37): methanol (15:85) UV (270 nm) Flow rate: 1.0 mL.min-1 Temperature: ambient [46]
10. Drug analysis Hypersil Phenyl 2 (250 × 4.6 mm, 5 μm) 0.02 M potassium dihydrogen phosphate, methanol, and triethylamine (25:75:0.2 v/v), pH 6.0 ± 0.1 was adjusted by addition of 10 orthophosphoric acid UV (271 nm) Flow rate: 1.0 mL.min-1 Temperature: room [47]
11. Everted gut sac (Rat) Shim-pack VP-ODS (150 × 4.6 mm, 5 μm) Acetonitrile: Methanol: water: glacial acetic acid (40:35:25:0.1 v/v), pH 6.8 UV (255 nm) Flow rate: 1.0 mL.min-1 Temperature: ambient [48]
Table 2: An updated account on various HPLC analysis employed for the estimation of Candesartan cilexetil.