S. No. Type of Media HPLC Column HPLC Mobile Phase Mass Analyzer Chromatographic conditions Reference
1. Rat plasma and bile AM-312 (ODS, 150 × 6 mm, 5μm) Water-acetonitrile-trifluoroacetic acid (70 : 30: 0.1v/v) Finnigan MAT (QqQ) Flow rate: 1.0 mL.min-1 Temperature: ambient [52]
2. Rat plasma ACE C18 column (50 × 4.6 mm, 5 μm) 10 mM ammonium acetate:acetonitrile (20:80, v/v) MDS Sciex API 4000 (Q) Flow rate: 0.5 mL.min-1 Temperature: 40 ± 2°C Detection: PDA (254 nm) [54]
3. Human plasma Synergy Polar RP (150 × 2 mm, 4μm) Solvent A: 0.1 formic acid (v/v) with 1 mmol/L ammonium formate Solvent B: acetonitrile: 0.1 formic acid 95:5 (v/v) with 1 mmol/L ammonium formate API 365 (QqQ) Flow rate: 0.25 mL.min-1 Temperature: 40°C [55]
4. Rabbit eyes Capcell Pak (4.6 × 250 mm, 5 μm) Acetonitrile and water (0.1 formic acid) 6410 (QqQ) Flow rate: 1.0 mL.min-1 Temperature: room [57]
5. Stability studies Luna C18 (150 × 4.6 mm, 5 μm) Acetonitrile and potassium dihydrogen orthophosphate buffer (pH 2.8; 0.01M) in a gradient mode MicrOTOF-Q (TOF) Flow rate: 1.0 mL.min-1 Temperature: room Detection: PDA (254 nm) [58]
6. Impurity profile Kromasil cyano column (250 × 4.6 mm, 5 μm) Water adjusted to pH 3.0 with TFA (A) and acetonitrile (B) in a gradient mode PolarisQ (IT) Flow rate: 1.0 mL.min-1 Temperature: room Detection: PDA (210 nm) [59]
7. Human plasma BEH C18, 1.7 μm column (analysis performed with Acquity UPLC) 0.1(v/v) formic acid in Distilled water and 0.1(v/v) formic acid in acetonitrile (60: 40, v/v) Micromass Quattro Premier XE Flow rate: 0.4 mL.min-1 Temperature: room [60]
8. Rat plasma Zorbax SB-C18 (150 × 4.6 mm) with a C18 guard column (4 × 3.0 mm, Phenomenex) Methanol and 5 mM ammonium acetate (50:50 v/v) MDS Sciex API 4000 Flow rate: 0.6 mL.min-1 Temperature: room Detection: PDA (254 nm) [61]
Q: Single quadrupole, QqQ: Triple quadrupole, IT: Ion trap, TOF: Time of flight, Q-TOF: quadrupole-time of flight
Table 3: An updated account on various LC-MS/MS analysis employed for the estimation of Candesartan cilexetil.