Parameters
Dual wavelength method
Q-analysis method
MCR method
EXRSM
ALP
BENZ
ALP
BENZ
ALP
BENZ
ALP
BENZ
λ(nm)
Difference in absorbance between 253 & 274.4 nm
Difference in absorbance between 238.2 & 261.2 nm
245.8 nm(λ
iso
) and 250(λ
max
)
Peak amplitudes(233.4 & 257.4 nm)
Peak amplitudes(228.4 & 252.6 nm)
250 nm(λ
max
)
238 nm(λ
max
)
Beer’s law range
5-60 µgml
-1
5-20 µgml
-1
5-60 µgml
-1
5-20 µgml
-1
5-50 µgml
-1
5-25 µgml
-1
5-50 µgml
-1
5-20 µgml
-1
Regression equation
Y=0.0368x-0.0126
Y=0.0438x-0.1063
C=(Q
m
-0.8055/1.0639-0.8055)× A/0.0484
C=(Q
m
-1.0639/0.8055-1.0639)× A/0.0457
Y=0.0591x-0.0004
Y=0.1535x-0.2765
Y=0.0517x-0.0064
Y=0.0664x-0.0967
Correlation coefficient
0.9999
0.9998
--
--
0.9999
0.9999
0.9998
0.9999
Accuracy
99.75
99.67
99.34
99.72
99.88
100.04
99.11
100.03
Precision
Repeatability
1.293
1.286
1.463
0.924
1.317
0.727
0.753
0.598
Intermediate precision
0.528
1.050
1.092
1.199
0.433
0.551
0.513
0.286
Table 1:
Linear regression and analytical parameters of the proposed methods for determination of Allopurinol and Benzbromarone.