Parameters TLC-method Fluorimetric method
Linearity range 0.5-2.5 µg/spot 0.1-3.6 µg/ml
Accuracy Mean ± RSD% 100.79 ± 0. 65%. 100.39 ± 1.12
Precision (n=9)  Mean ± RSD%
aIntraday
99.92 ± 0.44 100.29 ± 0.44
bInterday 100.65 ± 0.95 100.03 ± 1.07
Regression parameters
aSlope ± SE
1249 ± 16.91 115.2 ± 0.58
bIntercept ± SE 728.8 ± 30.86 18.10 ± 1.43
cCorrelation coefficient ± SE of estimation 0.9994 ± 20.07 0.9995 ± 1.26
LOD 0.001 0.013
LOQ 0.003 0.038
Table 1: Regression parameters and method validation results obtained by applying the proposed methods for determination of zaleplon.