Densitometric method (µg/spot) Spectrofluorimetric method (µg/ml)
Intact Degradations R% Intact Degradations R%
2.4 0.1 99.1 3.2 0.4 100.5
2.1 0.4 99.03 2.8 0.8 100.6
1.5 0.5 100.5 2 1.6 100.8
1.2 1.3 99.6 0.8 2.8 100.1
0.9 1.6 100.9 0.4 3.2 100.9
0.5 2 101.4 0.1 3.5 101.7
Mean ± SD 100.29 ± 0.96 Mean ± SD 100.77 ± 0.54
Table 2: Determination of zaleplon in mixtures with its acidic degradation products by the proposed methods.