| |
Level |
K’ |
T |
(Rs) |
| A: pH of mobile phase |
| 2.6 |
-0.2 |
2.8 |
1.22 |
3.4 |
| 2.8 |
0 |
2.13 |
1.23 |
3.4 |
| 3 |
0.2 |
2.2 |
1.4 |
3.2 |
| Mean ± S.D (n=6) |
2.13 ± 0.3 |
1.23 ± 0.020 |
3.4 ± 0.1 |
| B: Flow rate (mLmin-1) |
| 0.8 |
-0.2 |
2.1 |
1.45 |
3.32 |
| 1 |
0 |
2.3 |
1.44 |
3.36 |
| 1.2 |
0.2 |
2.4 |
1.42 |
3.37 |
| Mean ± S.D (n=6) |
4.3 ± 0.212 |
1.44 ± 0.015 |
3.36 ± 0.026 |
| C: Percentage of water in mobile phase (V/V) |
| 25 |
-5 |
2.6 |
1.42 |
3.38 |
| 30 |
0 |
2.3 |
1.43 |
3.36 |
| 35 |
5 |
2.5 |
1.46 |
3.33 |
| Mean ± S.D (n=6) |
2.36 ± 0.070 |
1.43 ± 0.025 |
3.36 ± 0.025 |
| C: Wavelength (nm) |
| 225 |
-5 |
2.5 |
1.42 |
3.38 |
| 230 |
0 |
2.3 |
1.43 |
3.36 |
| 235 |
5 |
2.4 |
1.45 |
3.32 |
| Mean ± S.D (n=6) |
2.3 ± 0.070 |
1.43 ± 0.015 |
2.36 ± 0.030 |
| K’= Capacity factors, N = Theoretical plates, T = Tailing factor Rs=Resolution |
|