Level of Structural Characterization |
Analytical information obtained |
Primary Structure |
Protein sequence
Nucleic acid sequence
Amino acid composition
Apparent molecular weight
Observed molecular mass
Post-translational modifications
Phosphorylation .
Glycosylation (monosaccharide composition) |
Secondary Structure |
Polypeptide chains
Peptide fragments
Disulfide bond linkages
Glycosylation (oligosaccharide structure)
lsoforms (e.g. glycoforms) |
Tertiary Structure |
Receptor binding
Epitope recognition
Cell modulator release
Cell differentiation effect
Replication competence
Apoptosis |
Product + Ligand Conjugate Molar ratios of ligand: product
(e.g. PEG, chemical or Ligand binding sites biological moeity)