Biomolecular Assay Type |
Potential Batch to Batch Variability |
Colorimetric |
Unique buffer components
Chromogenic reagent
Commercial kit active components |
Enzymatic |
Unique buffer components
Substrates
Enzymes
Cofactors
Detection reagents
Commercial kit active components |
Chromatographic |
Unique buffer components
Labile mobile phase solvents
Chromatography column resin
Derivatization or conjugation reagents |
Electrophoretic |
Unique electrode buffers
Gel matrix reagents
Sample treatment reagents
Staining reagents
Commercial kit components |
Immunological |
Primary antibodies
Secondary antibodies
Conjugated antibodies
Blocking reagents
Detection reagents
Commercial kit active components
Plastic cuvettes or micro titer plates |
Ligand Binding |
Unique buffer components
Target receptor
Target ligand
Detection reagents
Commercial kit active components
Plastic cuvettes or microtiter plates |
Cell Based Bioassay |
Cell seed stock (homogeneity and viability)
Cell culture (passage number and density)
Media components
Growth factors
Antimicrobial agents
Harvest reagents (e.g., trypsin)
Cell reactants (e.g., induction compounds)
Plastic flasks or plates |