Figure 2: Nucleotide sequence of the 5'-flanking region of the human SIRT1 gene. The nucleotide sequence of the 0.4-kb fragment obtained by PCR is shown. The most upstream of the cDNA (NM_012238.41) is designated nucleotide +1. Putative transcription factor-binding sites (TFSEARCH score>85) are shaded or indicated by dotted arrows. Bold italic characters represent the cDNA sequence of the SIRT1 gene (Gene ID: 23411).