Figure 3: Effects of natural compounds on the promoter activities of the human SIRT1 and TERT genes. (A) The Luc reporter plasmids, pGL4-SIRT1 and pGL4- TERT [13,23], were transfected into HeLa S3 cells as described under Materials and Methods. After 24 h of transfection, cells were treated with Quercetin (100 μM), Rsv (10 µM), and pine cone LCC (100 µg/ml), then harvested after a further 24 h incubation. (B) Similar experiment as in (A) was performed with 0 to 100 µg/ml of the pine cone LCC. The results show relative Luc activities of the indicated Luc reporter plasmid-transfected cells relative to that of non-treated cells. The values are the mean ± SD of four independent assays.