Figure 4: Changes in cell cycle and the expression of some relevant proteins by sanazole treatment. Cell cycle analyses in treated MES-SA cells showing G1 arrest after 24 h treatment with sanazole at 2, 5 and 10 mM (A), and after 48 h treatment with sanazole at 0.5, 1, 2 and 5 mM (B). In treated MES-SA/DX5v cells, S-phase arrest was observed after 24 h treatment with sanazole at 2, 5 and 10 mM (C), and after 48 h treatment with sanazole at 0.5, 1, 2 and 5 mM (D). Asterisks (*) indicate statistical significance (p<0.05). Western blot analysis shows the expression of p53 and p21 proteins following a 24 h-incubation period with different doses of sanazole (E).