Figure 6: Intracellular ROS elevation by sanazole treatment. Sanazole was added at different concentrations to cultured MES-SA and MES-SA/DX5v. Samples were collected at 6 h (A&C) and 24 h (B&D). Control and treated samples were analysed flow cytometrically for superoxide anion (O2-.) elevation using hydroethidine (HE) (A&B), and for peroxides elevation using dichlorofluorescein acetate (DCFH-DA) (C&D).