| Time(min) |
Event |
Conditions |
| 0.0 |
Sample injection |
Na-E buffer,48° C |
| 8.5 |
Start temp.gradient |
48° C to 60° C in 8 min |
| 24.5 |
Buffer change |
Na-E to Na-F |
| 41.0 |
Buffer change |
Na-F to Na-D |
| 78.0 |
Reagent pump |
Ninhydrin to water |
| 79.0 |
Buffer change |
Na-D to Na-R |
| 80.0 |
Buffer change |
Na-R to Na-E |
| 82.5 |
Temperature change |
60° C to 48°C |
| 84.0 |
Reagent pump |
Water to ninhydrin |
| 97.0 |
Recycle (Start next run) |
|
Buffer pump: 16 mL/h
Reagent pump: 8 mL/h |