| Year |
Scientist |
Research |
| 1958 |
Kslant |
First describing a kind of methods of extracting the polarity of steroids in the blood dialysis. |
| 1961 |
Gaddum |
Creating the push-pull type perfusion sampling method and applied for monitoring the brain nerve chemicals[4]. It was widely used in many parts of the brain neurotransmitters detection. |
| 1966 |
Bito |
First reported by using the semipermeable membrane to investigate the dog blood plasma and brain of the free amino acids and other liquid electrolytes for sampling[5]. |
| 1972 |
Yale University, Delgado |
First time using the brain microdialysis push-pull perfusion technique to investigate the neurotransmitter dopamine release from the monkey brain and this technique is established in the brain microdialysis technology in the neuroscience and brain research field.
The perfused dialytrodes were invented in 1972 helped to overcome the microdialysis method limitations[6]. |
| 1974 |
Urgestedt |
First time establishing the in vivo microdialysis technology applied in the living animals.
A “hollow fiber”-a diameter 200~300µm tubular semipermeable membrane was created to improve the dialysate concept.
Conducted the microdialysis technology to investigate the central dopamineneurons release from the rat brain[7]. |
| In the mid 1980s |
|
This method was widely used in the research on the drug distribution and metabolism of tissue in the body.Recently, it was used in the traditional Chinese medicine and acupuncture therapeutics. |