Parameters Conc  μg /ml Accuracy % RSD % RE %
Short term
At 25°C, 24 hr
0.5
1.5
2
108
110.6
96.5
2.3
1.16
7.7
7.8
10.6
-3.7
Long term
At -20°C, 30 days
0.5
1.5
2
106
105.3
104
3
2.3
6.1
6.8
5.6
4
Three freeze-thaw cycles
Thawed at 25°C for 2 h, refrozen 24h
0.5
1.5
2
94
106.6
98.5
1.8
0.75
8.9
-5.1
7.2
-1.7
Table 2: Summary of stability of ATM in human plasma at varying condition by the proposed UPLC method.