Parameter UPLC method   Authentic sample Spiked plasma Derivative synchronous spectroscopy FDSFS
Linearity range μg /ml 0.1-35 0.1- 4 1-12
Regression data:
n 7 7 7
Slope ( b) 115.12 2.01 0.298
SD of slope 0.65 0.014 0.71
Intercept ( a ) -12.328 0.0158 0.3122
SD of intercept 0.92 0.02 0.03
Correlation coefficient r 0.9998 0.9998 0.9978
Standard error SE % 0.35 2.23 0.011
Recovery ± SD %
Drug substance
Drug product
Added standard
98.55 ± 0.78
99.75 ± 0.93
99.98 ± 0.83
95.6 0 ± 5.70
94.33 ± 7.40
98.32 ± 0.66
98.66 ± 0.86
99.11 ± 0.71
Precision ± RSD %
Intra – day
Inter – day
98.80 ± 0.56
100.1 ± 0.39
100.60 ± 3.90
103.2 ± 5.30
98.75 ± 0.75
98.82 ± 0.65
LOD μg /ml 0.03 0.03 0.33
LOQ μg /ml 0.10 0.10 1
Y=a+b C, where C is the concentration of the drug in μg/ml and Y is the peak ratio of drug/I.S for the UPLC method or fluorescence intensity for fluorometric method.
Table 3: Validation of the proposed UPLC method and derivative synchronous fluorescence spectroscopy for the determination of ATM.