S. No Concentration μg mL-1 Mean μg mL-1 % Recovery Relative error (%) CV (%)
1 1 0.999 99.89 0.15 1
2 2 2.005 100.23 -0.5 1.19
3 3 3.03 101 -1 0.79
4 4 3.965 99.12 0.05 0.64
5 5 5.005 100.1 -0.91 0.12
Correlation coefficient (r2)=0.999
LLOD =0.04
LLOQ =0.12
Table 4: Recovery of pregabalin from human serum (n=3).