Figure 3: Induction of loss of mitochondrial membrane potential (MMP or ΔΨm) by F. solani organic extract. HeLa cells were treated for 24 h with 2.5 μg/mL (D), 5 μg/mL (E), 10 μg/mL (F), 25 μg/mL (G) and 50 μg/mL (H) of F. solani organic extract or the positive control agent 2,4 DNP (C). A and B represent experimental controls of untreated cells alone and untreated cells + C-1 stain, respectively. ΔΨm was measured by BD FACScan flow cytometer as described under “Materials and Methods”. One of the three independent experiments is shown.