Robustness (% assay mean ± SD)
  L-Lysine hydrochloride LCLT
Change in flow rate    
0.3mL/min (n=3) 100.12 ± 1.147 99.50 ± 1.203
0.7mL/min (n=3) 99.98 ± 0.901 100.01 ± 0.657
Change in detection wavelength    
212nm (n=3) 100.69 ±1.195 100.44 ± 1.061
216nm (n=3) 100.81 ± 1.047 100.55 ±0.935
Change of column    
Merck (n=3) 100.83 ± 1.032 99.48±0.900
Teknokroma (n=3) 100.72 ± 1.177 99.43 ± 1.085
Change in column temperature    
23°C (n=3) 99.87 ± 0.772 100.63 ± 0.846
27°C (n=3) 101.85 ± 0.873 101.51 ± 0.991
Table 3: Assay results for L-Lysine hydrochloride and LCLT.