Item Amlodipine besylate Losartan potassium Valsartan Atorvastatin calcium
Linear range (µg/ml) 2-12 10-60 16-96 4-24
Detection limit (µg/ml) 0.03 0.18 0.15 0.007
Quantitation limit (µg/ml) 0.1 0.54 0.45 0.024
Regression data        
N 5 5 5 5
Slope (b) 64.62 75.39 64.49 70.96
Standard deviation of the slope 0.71 0.1 0.065 0.69
Intercept (a) 6.0 8.39 30.19 33.99
Standard deviation of the intercept 0.62 4.09 2.95 0.17
Correlation coefficient ® 0.9998 0.9997 0.9998 0.9998
Standard error of regression 0.07 0.40 0.47 0.17
(Y=a+bC, where C is the concentration of the compound (µg/ml) and Y is the drug peak area)
Table 4: Calibration data was resulted from method validation of Amlodipine besylate, Losartan potassium, Valsartan and Atorvastatin calcium respectively.