Independent Variable Dependent Variables
Runs Batches X1 X2 X3 Y1 (ACTUAL) Y1(PREDICTED)           
1 B1 0 1 -1 0.352   0.67
2 B2 -1 -1 0 0.476 0.24
3 B3 1 1 0 0.621 0.25
4 B4 -1 1 0 0.492 0.23
5 B5 1 -1 1 0.758 0.11
6 B6 0 -1 1 0.675 0.67
7 B7 -1 0 -1 0.201 0.697
8 B8 1 -1 0 0.453 0.23
9 B9 0 0 0 0.432   0.67
10 B10 0 0 0 0.408 0.24
11 B11 0 0 0 0.421   0.24
12 B12 0 1 1 0.871 0.16
13 B13 0 -1 -1 0.286 0.23
14 B14 0 0 0 0.465 0.24
15 B15 -1 0 1 0.568 0.11
16 B16 -1 -1 0 0.432 0.16
17 B17 1 0 -1 0.253 0.20
Table 2: X1=FC Reagent (ml) X2=Sodium carbonate (ml) X3=Drug Conc. (μg ml-1) Y1=Absorbance.