Run
ACN
Flow rate (ml)
pH
Area
Retention time
Capacity factor
Actual value (Mean ± SD) (n=3)
Predicted value
Actual value (Mean ± SD) (n=3)
Predicted value
Actual value (Mean ± SD) (n=3)
Predicted value
1
80.00
0.80
7.00
401423 ± 6248
401603
4.03 ± 0.062
4.01
2.36 ± 0.036
2.37
2
80.00
1.00
6.00
422691 ± 6023
423410
3.68 ± 0.052
3.69
2.05 ± 0.031
2.06
3
90.00
1.00
5.00
378411 ± 6157
379013
5.13 ± 0.065
5.11
3.24 ± 0.051
3.24
4
90.00
1.20
6.00
349467 ± 4529
349119
4.48 ± 0.061
4.50
2.73 ± 0.044
2.74
5
90.00
0.80
6.00
359129 ± 5488
359312
4.62 ± 0.064
4.63
2.84 ± 0.041
2.85
6
90.00
1.00
7.00
427143 ± 6759
426804
4.52 ± 0.066
4.51
2.78 ± 0.043
2.79
7
70.00
0.80
6.00
285321 ± 4581
285711
3.74 ± 0.051
3.72
2.11 ± 0.029
2.12
8
80.00
1.00
6.00
422658 ± 6589
423407
3.68 ± 0.058
3.69
2.06 ± 0.029
2.06
9
70.00
1.00
5.00
378854 ± 6772
379209
3.43 ± 0.054
3.44
1.84 ± 0.025
1.85
10
80.00
0.80
5.00
412894 ± 7025
412217
4.09 ± 0.056
4.07
2.41 ± 0.037
2.43
11
80.00
1.00
6.00
424759 ± 6855
423434
3.69 ± 0.055
3.68
2.07 ± 0.027
2.06
12
80.00
1.20
7.00
401267 ± 5496
402006
3.72 ± 0.061
3.71
2.15 ± 0.031
2.15
13
70.00
1.00
7.00
305821 ± 3544
305308
4.03 ± 0.049
4.01
2.35 ± 0.033
2.36
14
80.00
1.20
5.00
417741 ± 6554
417510
3.69 ± 0.052
3.68
2.07 ± 0.027
2.08
15
70.00
1.20
6.00
301623 ± 3624
301505
3.17 ± 0.039
3.18
1.65 ± 0.021
1.66
Table1:
Observed values of responses for Box–Behnken design (Design Expert 8.0.7.1).