Conc. (ng/ml) Stability condition Conc. (ng/ml) (Mean ± SD) % Drug Remaining % RSD
LQC (20 ng/mL) Initial (0 h) 19.71 ± 0.31 100 1.57
Short-term stability (25°C, 24 h) 19.64 ± 0.34 99.64 1.73
Long-term stability (−80°C, 30 days) 19.58 ± 0.27 99.34 1.37
Three cycles freeze–thaw stability (three cycles, −20°C to 25°C) 19.50 ± 0.29 98.93 1.48
Post-preparative stability (10°C, 24 h) 19.46 ± 0.28 98.73 1.43
HQC (1000 ng/mL) Initial (0 h) 992.76 ± 16.58 100 1.67
Short-term stability (25°C, 24 h) 984.89 ± 15.92 99.20 1.61
Long-term stability (−80°C, 30 days) 978.56 ± 16.23 98.56 1.65
Three cycles freeze–thaw stability (three cycles, −20°C to 25°C) 970.45 ± 16.04 97.75 1.65
Post-preparative stability (10°C, 24 h) 966.51 ± 15.94 97.35 1.64
Table 7: Summary of ex vivo stability assessment (n=6).