Compounds Acetone MeOH Acetone/MeOH 50/50 MeOH/ACN/citric acid (pH=4) MeOH/ACN/phosphoric acid (pH=3.1)
ANTIBIOTICS
sulfanilamide 46 ± 1.6 84 ± 4.4 101 ± 20.7 70 ± 1.8 73 ± 12.1
sulfadiazine 18 ± 0.2 91 ± 2.1 90 ± 5.0 106 ± 0.6 89 ± 1.2
sulfathiazole 19 ± 0.3 78 ± 2.2 96 ± 4.6 110 ± 1.21 82 ± 0.7
sulfameter 37 ± 1.3 91 ± 4.3 100 ± 8.2 109 ± 0.3 92 ± 4.1
trimethoprim 9 ± 0.1 15 ± 0.8 23 ± 2.0 79 ± 2.4 82 ± 1.3
sulfadimidine 64 ± 0.3 90 ± 2.3 94 ± 2.8 109 ± 1.1 89 ± 0.5
sulfabenzamide 17 ± 0.3 75 ± 1.0 87 ± 8.3 110 ± 3.3 99 ± 2.0
sulfadimethoxine 57 ± 1.5 88 ± 2.4 99 ± 6.8 110 ± 8.7 94 ± 3.5
erythromycin 4 ± 0.2 16 ± 2.0 16 ± 0.6 40 ± 18.8 33 ± 2.1
tylosin 7 ± 0.2 42 ± 2.6 65 ± 7.6 95 ± 8.5 86 ± 2.9
roxithromycin 7 ± 0.4 64 ± 2.0 48 ± 2.3 100 ± 3.8 97 ± 2.5
penicillin G nd 6 ± 1.4 14 ± 0.1 82 ± 1.23 75 ± 0.3
dicyclanil 60 ± 0.1 62 ± 3.8 63 ± 3.0 82 ± 1.9 102 ± 9.3
florfenicol 83 ± 5.0 91 ± 6.7 105 ± 6.0 109 ± 1.1 96 ± 6.3
STEROID HORMONES
androstenedione 75 ± 3.0 92 ± 7.2 96 ± 8.1 97 ± 0.7 99 ± 5.6
testosterone 76 ± 1.5 112 ± 2.8 104 ± 18.9 100 ± 3.8 101 ± 4.2
progesterone 77 ± 1.0 135 ± 3.5 117 ± 22.9 91 ± 2.1 108 ± 13.7
norethindrone 81 ± 0.8 102 ± 0.9 109 ± 1.4 96 ± 5.8 100 ± 4.2
gestodene 69 ± 11.7 108 ± 14.1 96 ± 16.2 100 ± 2.5 101 ± 13.5
levonorgestrel 74 ± 2.6 98 ± 1.6 112 ± 5.4 99 ± 2.7 113 ± 20.8
estriol 59 ± 4.2 28 ± 1.9 33 ± 3.0 20 ± 1.8 8 ± 2.4
estrone 78 ± 3.7 89 ± 3.4 80 ± 3.6 60 ± 2.2 62 ± 3.5
17α-estradiol 75 ± 1.7 69 ± 2.9 73 ± 1.0 50 ± 2.0 47 ± 2.0
17b-estradiol 75 ± 1.2 90 ± 3.7 78 ± 5.2 50 ± 3.3 36 ± 4.4
17α-ethinylestradiol 69 ± 11.0 81 ± 1.3 70 ± 0.1 60 ± 1.8 64 ± 0.7
HUMAN CONTAMINANTS
sulfamethoxazole 48 ± 0.3 99 ± 3.2 102 ± 0.8 110 ± 2.2 100 ± 3.2
carbamazepine 90 ± 0.5 99 ± 1.9 97 ± 1.7 96 ± 1.6 101 ± 3.2
fluvoxamine 3 ± 0.1 38 ± 2.0 nd 60 ± 1.1 60 ± 18.9
paracetamol 40 ± 0.1 18 ± 0.4 17 ± 1.3 8 ± 0.2 4 ± 0.4
ibuprofen 1 ± 0.16 43 ± 7.3 68 ± 7.9 97 ± 7.0 98 ± 7.7
bisphenol A 100 ± 7.1 77 ± 5.7 70 ± 5.0 42 ± 1.7 43 ± 5.4
Table 1: PLE recoveries (%) for the 31 studied compounds and for the following solvents: acetone, MeOH, acetone/MeOH (50/50), MeOH/ACN/citric acid (pH=4) and MeOH/ACN/phosphoric acid + citric acid (pH=3.1).