Design Trials Levels (ml) Absorbance
PDAB reagent Ferric Chloride At λmax 425 nm
T-1 1 1 0.233
T-2 2 2 0.252
T-3 3 3 0.276
T-4 1 4 0.291
T-5 2 1 0.312
T-6 3 2 0.326
T-7 4 3 0.335
T-8 5 4 0.348
T-9 6 5 0.352*
T-10 4 6 0.355*
T-11 5 7 0.356*
T-12 6 8 0.349*
T-13 7 5 0.347
T-14 8 6 0.341
T-15 9 7 0.338
T-16 7 8 0.331
T-17 8 9 0.324
T-18 9 10 0.320
T-19 10 9 0.317
T-20 10 10 0.315
Aristech (*) value indicated the significant variable (better) optimum level response
Table 1: Generated Array Trials At Diverse Levels (1 To 10).